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American Society of Microbiology ABMM Sample Questions:

1. A microbiology laboratory receives a bronchoalveolar lavage sample from a patient with suspected ventilator- associated pneumonia. Quantitative culture yields >10^5 CFU/mL of a Gram-negative rod. Identification reveals Stenotrophomonas maltophilia. The MOST significant resistance mechanism commonly associated with this organism is:

A) Target site modification in DNA gyrase.
B) Production of extended-spectrum beta-lactamases (ESBLs).
C) Intrinsic resistance mediated by efflux pumps and specific enzymes.
D) Ribosomal methylation leading to macrolide resistance.

2. Transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease (CJD) in humans and Bovine Spongiform Encephalopathy (BSE) in cattle, are caused by infectious agents composed primarily of:

A) Slow-growing bacteria lacking a cell wall
B) Viroids (small, circular RNA molecules)
C) Aberrantly folded host-encoded prion proteins (PrP<sup>Sc</sup>)
D) Defective viral particles requiring a helper virus

3. The gut microbiome has been implicated in the pathogenesis of autoimmune diseases. Dysbiosis, a disruption in the composition and function of the gut microbiota, can lead to increased intestinal permeability and the translocation of microbial products that trigger inflammatory responses. Which of the following bacterial components is a potent activator of the innate immune system and has been linked to the development of autoimmunity?

A) Lipopolysaccharide (LPS)
B) Lipoteichoic acid (LTA)
C) Peptidoglycan
D) Flagellin

4. According to the current CDC-recommended laboratory algorithm for diagnosing HIV infection in adults and children aged >18 months, what is the recommended initial screening test?

A) HIV-1 p24 antigen assay only
B) HIV RNA nucleic acid test (NAT)
C) HIV-1/HIV-2 antigen/antibody combination immunoassay
D) HIV-1/HIV-2 antibody differentiation immunoassay

5. A patient develops a febrile illness with a diffuse petechial rash after a camping trip. Serological testing reveals a four-fold rise in antibody titer to Rickettsia rickettsii. The vector responsible for transmitting this organism is a:

A) Tick
B) Louse
C) Flea
D) Mosquito

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